ISLR and breast cancer: To more comprehensively identify whether ISLR is a redox sensor of ROS, we treated four different cell lines, which including 4T1 (breast cancer cells), A549 (lung cancer cells), HEK293T (embryonic human kidney cells), and C3H10T1/2 (mesenchymal progenitor cells) cells with H2O2, a natural ROS produced by mitochondria.