TNFRSF14 and neoplasm: HVEM+ K562 cells and HVEM− Hek293 were treated first with 20 ng/100 μL of HVEM blocker using 1 × 105 cells for 2 h before being washed and then co-cultured with CFSE-labeled CD4+ T cells at a ratio of one tumor or healthy control cell to ten CFSE CD4+ T cells.