Since the disruption of HAO1 through encoding the glycolate oxidase (GO) enzyme that synthesizes glyoxylate has proven efficient and safe in severe PH1 patients, a team has developed a way to knockout HAO1 by systemic delivery of AAV8-SaCas9 nuclease and a sgRNA in hepatocytes of the mouse model Agxt1−/− [137]. This evidence concerns the gene HAO1 and primary hyperoxaluria type 1.