To treat hemophilia A and B, an LNP-based approach was developed to deliver SpCas9 nuclease mRNA and sgRNA to disrupt the expression of Serpinc1, encoding the anticoagulant protein antithrombin (AT), in the liver of hemophilic mouse models F8I22I and F9Mut [74]. The gene discussed is SERPINC1; the disease is hemophilia A.