Next-generation sequencing in a cohort of lung adenocarcinoma patients undergoing primary tumor resection found that increased intraoperative opioid dosage was associated with poorer overall survival and that this association was amplified in patients with mutations in the CDKN2A tumor suppressor gene, the alterations in the Hippo and Wnt pathways, combined with increased doses of opioid drugs, are associated with a reduced recurrence rate of tumors (61). This evidence concerns the gene CDKN2A and lung adenocarcinoma.