In conclusion, our study provisionally confirms that DB + B can improve the protective benefit of IR brain injury by effectively regulating intestinal flora including o_Pseudomonadales, s_Bacteroides_caecimuris, o_unidentified_Bacilli, f-Pseudomonadaceae, and g-Pseudomonas, mainly regulating serum metabolites including lysoPCs and lysoPEs thus inhibiting TLR4/MyD88/NF-κB and IL-17 signing pathway to reduce inflammatory reactions. Here, TLR4 is linked to injury.