POLE and colorectal carcinoma: Finally, we considered POLE genetic stratification: in both CRC datasets, all references led to effective discrimination of POLE-mutated from POLE wild-type CRCs (Wilcoxon rank sum test, C2vsTS, C2vsC3, C3vsTS, P < 2e-16) (preclinical dataset: respectively ΔPOLE = 0.59, ΔPOLE = 0.47, ΔPOLE = 0.51; clinical dataset: ΔPOLE = 0.59, ΔPOLE = 0.59, ΔPOLE = 0.61, Fig. 5E–F).