Gene set analysis revealed an upregulation of K-RAS signaling (Supplementary Fig. 5A), glutamatergic synapse (Supplementary Fig. 5B), and epileptic encephalopathy (Supplementary Fig. 5C) when comparing TSC2 mutant (TSP8-15) versus control (CC3) neural cells for RNA expression differences. Here, KRAS is linked to Epileptic encephalopathy.