Overall, these data illustrate that ECs express GNG enzymes, at higher levels upon glucose deprivation, and that PCK2 regulates in ECs a truncated GNG (reverse glycolysis) pathway, which does not serve the purpose of glucose production and secretion (as in liver and kidney10) or nucleotide biosynthesis via the PPP (as in cancer cells31,32). Here, PCK2 is linked to cancer.