Previously, our and other research teams have demonstrated that Dox treatment can cause cardiomyocyte autophagic flux blockage and apoptosis, leading to cardiac dysfunction and heart failure.20 Interestingly, cardiomyocyte-specific knockout of Ddx17 also exacerbated Dox-induced autophagic flux blockage and apoptosis in myocardial tissue in vivo (Supplementary Fig. 5a–f). Here, DDX17 is linked to heart failure.