To investigate the biological function of eIF3a in CRC, endogenous eIF3a mRNA in SW620 and DLD-1 cells was silenced via transfection with si-eIF3a (Figure 2a), which led to a decrease in eIF3a protein levels (p < .01), as confirmed via western blotting (Figure 2b,c). Here, EIF3A is linked to colorectal carcinoma.