We further measured TNF-α expression levels by IHC staining in the prostates of PBCrePten–/–Klf5KR/KR and PBCrePten–/–Klf5+/+ mice and confirmed that Klf5KR knockin significantly induced TNF-α expression in Pten-deficient mouse prostate cancer (Figure 6F). This evidence concerns the gene KLF5 and prostate carcinoma.