To determine whether deAc-KLF5 affects TNF-α secretion in cancer cells, we measured the expression levels of TNF-α in DU 145 prostate cancer cells with KLF5WT and KLF5KR in different culture conditions, including with cancer cells alone, cancer cells treated by CAF CM, and cancer cells cocultured with CAFs (Figure 6G). This evidence concerns the gene TNF and cancer.