Utilizing multiplex immunohistochemistry (mIHC), tissue microarrays (TMAs) representing a cohort of BE, low-grade dysplasia (LGD), high-grade dysplasia (HGD), and EAC were stained for CD3-ε (T cell), CD3-ζ (T cell), CD8 (effector T cell), CD163 (macrophages), FoxP3 (Tregs), pan-Cytokeratin (PanCK: tumor “target” cells), and PD-L1 antibodies. Here, CD163 is linked to neoplasm.