reported that shRNA‐mediated knockdown of Akt3 expression significantly inhibits the growth of MDA‐MB‐231 or MCF10DCIS cells in 3D cultures and in tumor‐treated animal models, in comparison to shRNA suppressing of Akt1 and Akt2 expression.[38] In contrast, PTEN‐deficient TNBC spheroids are also dependent on Akt2‐mediated survival signaling.[39] Interestingly, although Akt1 promotes tumor induction, it has been shown to inhibit migration, invasion, and metastasis of breast cancer cells.[40, 41] Therefore, depleting Akt1 may have undesired effects of enhancing metastatic dissemination. This evidence concerns the gene AKT2 and breast cancer.