Our results indicated an upregulation of SLC31A1, CD274, ATOX1, MTF1, DLD, VEGFA, DLST, COX11, DLAT, and GLS, which aligns with previous bioinformatics analysis and the expression profile of CRG cluster B. These findings suggest the involvement of CRGs in the immune-mediated inflammatory response of sepsis-associated ALI. Here, ATOX1 is linked to acute respiratory distress syndrome.