To elucidate the mechanisms underlying the induction of PD-L1 expression by PAI-1, we prepared tumor cell lines deficient in uPAR (uPAR-KO; Supplementary Figure S3A) or LRP-1 (LRP1-KO; Supplementary Figure S3B) using the CRISPR/Cas9 system. Here, CD274 is linked to neoplasm.