AKT1 and cancer: Since clemastine and hyperthermia sensitized OS cells to apoptosis and inhibited cell proliferation, we further analyzed the effect of clemastine and hyperthermia on AKT/mTOR signaling, since the AKT/mTOR signaling pathway is involved in cell survival and proliferation and is activated in various human cancers.27 U-2 OS and Saos-2 cells were exposed to clemastine and hyperthermia alone or in combination and expression of AKT/mTOR was analyzed by western blotting.