CD8 + T cells also referred to as cytotoxic T cells, play a crucial role in the immune system by recognizing and destroying cells that are infected or abnormal, including cancer cells.27 However, CD8 + T cells in the TNBC immune microenvironment are often affected by many different factors leading to anti-tumor dysfunction.28 Building on the results of the immune analysis, we conducted co-cultures of TNBC cells with PBMCs to investigate the potential involvement of KRT81 in immune suppression targeting CD8 + T cells. Here, CD8A is linked to cancer.