To elaborate further on Ki67 and insulin dual-stainings, we could not observe any significant difference in β-cell proliferation, but we observed a substantial reduction in levels of apoptotic Tunel Insulin cells, Death Receptor-5 activity, and cleaved caspase-3 and in AGE-treated β-cell compared to DM β-cell (Fig. 7C, D). Here, INS is linked to diabetes mellitus.