These findings offer compelling evidence that the protective mechanism of MTH involves the regulation of O-GlcNAcylation levels, which are consistent with the results presented in Sects. "MTH reduced myocardial infarction area and improved hemodynamic performance after ischemia–reperfusion injury in rats" and "MTH induces an increase in OGT-dependent O-GlcNAcylation levels in myocardial tissue". The gene discussed is OGT; the disease is ischemia.