To determine the expression and activity of p53 in Lmna-mutant myofibers, we differentiated primary myoblasts isolated from three different mouse models of striated muscle laminopathies, along with Lmna wild-type (Lmna WT) littermate controls, using an in vitro differentiation platform that allows for maintaining myofibers for 10 days (Fig. 1A) [34]. Here, LMNA is linked to laminopathy.