KLF2 is known to integrate hemodynamic and pro-inflammatory stimuli to maintain vascular homeostasis and integrity27,28 In snRNA-seq, most of the vascular endothelial subtypes showed downregulation of KLF2 in the IHD group compared to control samples (Figure 2I), suggesting the emergence of endothelial cell dysfunction in the cardiac vasculature, which has been shown to be critical in the initiation and progression of cardiovascular disease.29 The gene discussed is KLF2; the disease is myocardial ischemia.