CP and infection: At 20 dpai, green fluorescence and accumulation of CP were individually detected in the upper uninoculated leaves of YJM plants infected with WMV-GFP, WMV-GFP-P3NL35I-PIPO, and WMV-GFP-P3NI43V-PIPO, but they were not detected in that of WMV-GFP-P3NL35V-PIPO challenged plants (Fig. 5B and C, middle panel), suggesting the L35V mutation may disrupt the infection of WMV isolate GN.