In ChIP-Seq analyses from a recent study investigating BET-i in acute graft-versus-host disease (GVHD) (47), BRD4 was enriched at regulatory regions of RUNX3, PRDM1 (BLIMP1), and BATF in healthy donor T cells and enrichment was reduced following treatment with 10 nM OPN-2853 (approximately equivalent to 0.1 μM of OPN5; Supplemental Figure 17) (15). This evidence concerns the gene SPP1 and acute graft versus host disease.