GFAP and astrocytoma (excluding glioblastoma): As a first assessment of HIF-Clear applicability to disease models, we subjected an intact FFPE brain from an astrocytoma mouse model (see ‘Materials and methods’) to the HIF-Clear pipeline to label tumor cells (ASTS1CI-GFP positive astrocytes) and GFAP-positive astrocytes (Figure 7—video 1, Figure 7A and C).