Additionally, a study on nonalcoholic fatty liver disease revealed that diabetic mice with reduced insulin receptor (InsR) fed a methionine choline (MCD)-deficient diet exhibited impaired liver fat content and hepatic insulin signaling, leading to the accumulation of Forkhead box protein O1 (FoxO1) and subsequent induction of lysyl oxidase-like 2 (Loxl2). This evidence concerns the gene INS and metabolic dysfunction-associated steatotic liver disease.