Cytokines play a crucial role in regulating and activating asthma, and as ACO shares some characteristics with asthma, this study demonstrates that the ACO-Pep-3-EcTI, ACO-Pep-BbKI, ACO-Pep-BrTI, and ACO-DX treatment groups exhibited a positive response in controlling inflammation. This evidence concerns the gene KLK15 and asthma.