In addition, we performed RNA sequencing of tumor-associated macrophages (TAMs), splenic macrophage (SpM) and peritoneal macrophage (PM) in 4T1 tumor-bearing mice, and found that Fzd2 (Frizzled Class Receptor 2) and Kdr (Kinase Insert Domain Receptor) were barely expressed in macrophages, and that C1qbp was highly expressed in tumor-associated macrophages, while Vcan was not highly expressed in TAMs (Figure 2E). Here, VCAN is linked to neoplasm.