Immunohistochemical staining, western blot, qRT-PCR and ELISA analyses further confirmed that IL-4 was significantly down-regulated in the tumor tissues of Postn-/-Rag1-/- injected with IHH-4 cells compared to their Postn+/+Rag1-/- counterparts (Figure 5A-D). This evidence concerns the gene RAG1 and neoplasm.