characteristics of the organoids, similar to HCC tissue a significant upregulation in the expression of the invasion metalloproteases MMP-2, MMP-3, and MMP-13 were noticed in 3D (Mixed ) organoids compared to the 2D cultured HUH-7 cells (HUH7(2D)) and 3D (HUH-7). Here, MMP13 is linked to hepatocellular carcinoma.