Up-regulation of HCC genes c-Myc, TGF\documentclass[12pt]{minimal}\usepackage{amsmath}\usepackage{wasysym}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{amsbsy}\usepackage{mathrsfs}\usepackage{upgreek}\setlength{\oddsidemargin}{-69pt}\begin{document}$$\beta$$\end{document}, TCF4, RHOA, and IGF2 in the 3D Mixed organoids compared to the 3D HUH-7 ones suggests better modeling for increased cell proliferation and tumor growth leading to poor prognosis HCC tumors [79–83]. This evidence concerns the gene RHOA and hepatocellular carcinoma.