TNF and Miyoshi myopathy: ELISA analysis further revealed that although the levels of IL-10 and TGF-β1 remained unaffected by STING agonist-4 (as these cytokines are produced by Tregs), STING agonist-4 treatment significantly increased the production of IL-6, TNF-α and CXCL10 in MM cells when co-cultured with Tregs (Fig. 4D).