Additionally, induction of ANO1 expression by 5-Aza-CdR treatment increased cancer characteristics, but silencing of ANO1 expression by RNAi attenuated the treatment effects, suggesting 5-Aza-CdR-induced metastatic potential of LNCaP and DU145 cells was generated through its major impact on ANO1 expression. Here, ANO1 is linked to cancer.