To explore astrocytic ALKBH5 modulate depression-related behaviors via glutamatergic neuronal activity, we bidirectionally injected the AAV-CamkIIα-hM4D(Gi)-mCherry virus into the mPFC of Astrocyte cKO mice to specifically manipulate the activity of glutamatergic neurons. The gene discussed is CAMK2A; the disease is depressive disorder.