At this stage, there is an increase in the helper T-cell 1 (Th1) marker cytokine interleukin-17 (IL-17), suggesting an increase in Th1 cells, whereas interleukin-4 (IL-4) and interleukin-10 (IL-10) are not induced, suggesting that there are no significant changes in regulatory T cells or helper T-cell 2 (Th2) cells in the splenocytes of mice in the early stage of AP [63]. This evidence concerns the gene IL10 and alkaline phosphatase measurement.