For example, in a patient with organ-confined (pT2) prostate cancer and LN metastasis (patient #33, Fig. 2b and S3b), the samples demonstrated genomic complexity with FOXA1 and ATM frameshift mutations detected in some primary tumor and LN metastasis regions (P2, P3, P5, P6, LN1); an SPOP mutation was detected only in a GG1 primary tumor focus (P1); and RB1 loss and MYC gain were shared between the presumed dominant primary tumor regions and the LN metastasis focus (P2, P3, P6, LN1). This evidence concerns the gene SPOP and Familial prostate cancer.