RB1 and Familial prostate cancer: For example, in a patient with organ-confined (pT2) prostate cancer and LN metastasis (patient #33, Fig. 2b and S3b), the samples demonstrated genomic complexity with FOXA1 and ATM frameshift mutations detected in some primary tumor and LN metastasis regions (P2, P3, P5, P6, LN1); an SPOP mutation was detected only in a GG1 primary tumor focus (P1); and RB1 loss and MYC gain were shared between the presumed dominant primary tumor regions and the LN metastasis focus (P2, P3, P6, LN1).