When the selected variants were tested on the orthogonal test set (n = 67), the PRS was significantly increased in the responders (Wilcoxon rank sum test, p value = 0.00071, Fig. 7A), and exhibited a higher area under the receiver operating characteristic curve (AUC, ROC) than PD-L1 expression (Fig. 7B), as well as against tumor mutational burden (TMB) or microsatellite instability (MSI) as calculated from WES data (Supplementary Fig. 7). Here, CD274 is linked to neoplasm.