The ability of the discovered anti-αNTx VHHs to neutralize αNTx-mediated blocking of the muscle type nAChR function was evaluated using whole-cell patch clamp recordings of rhabdomyosarcoma cells endogenously expressing the nAChR, which were exposed to purified αNTx DH or the consensus toxin scNTx at a concentration resulting in approximately 80% inhibition (IC80) (Fig. 3B). Here, CHRNA4 is linked to rhabdomyosarcoma.