To identify potential pathogenic variants associated with hypotrichosis causative genes like KRT74, KRT25, KRT71, APCDD1, RPL21, SNRPE, CDSN, U2HR, EPS8L3, HR, DSG4, LIPH, LPAR6, DSC3, KRT25, LSS, TTMP, KRT86, KRT83, and KRT81, gene panel sequencing was used. The gene discussed is KRT25; the disease is hypotrichosis.