To identify potential pathogenic variants associated with hypotrichosis causative genes like KRT74, KRT25, KRT71, APCDD1, RPL21, SNRPE, CDSN, U2HR, EPS8L3, HR, DSG4, LIPH, LPAR6, DSC3, KRT25, LSS, TTMP, KRT86, KRT83, and KRT81, gene panel sequencing was used. This evidence concerns the gene LSS and hypotrichosis.