Here we have developed and characterized human iPSC derived ECs (iECs) and used these generated cells as a proof-of-concept model to study TIE2-related venous malformations (VMs). TIE2 (gene name TEK), is a member of the receptor tyrosine kinase subfamily and is almost exclusively expressed in vascular ECs [7, 8]. This evidence concerns the gene TEK and Venous malformation.