The enrichment analysis conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the differentially expressed genes were primarily involved in the metabolism of xenobiotics by cytochrome P450, retinol metabolism, drug metabolism-cytochrome P450, cell adhesion molecules, steroid hormone biosynthesis, and the nuclear factor kappa B (NF-κB) signaling pathway.<h4>Conclusions</h4>The highly bone-metastatic human lung adenocarcinoma cell line with GFP and luciferase double labeling was successfully established. The gene discussed is NFKB1; the disease is lung adenocarcinoma.