To explore the specific signal pathway in TERT mutant HCC cells in response to PLK1 inhibitor for TERT regulation, we made the following hypothesis: First, TERT promoter mutation induced differential expression of some genes; Secondly, PLK1 inhibitor selectively influenced the change of these genes in TERT mutant HCC cells; Thirdly, these special genes may directly interact with PLK1 or PLK1 downstream targets as well as TERT or its upstream regulators, since PLK1 is a kinase and TERT was regulated at translational level. Here, PLK1 is linked to hepatocellular carcinoma.