In an effort to investigate the impact of the lactylation of NSUN2 on its nuclear localization, immunofluorescence and protein nucleocytoplasmic separation were performed in CRC cells following the addition of lactic acid, and the results consistently showed no alternation in subcellular localization following lactic acid treatment (Figure 5L‐M). The gene discussed is NSUN2; the disease is colorectal carcinoma.