Overexpression (Additional file 3: Figure S1B) and knockdown (Additional file 3: Figure S1C) of LINC00330 in ESCC cell lines were achieved by transient transfection or lentiviral packaging and infection, followed by a series of gain-of-function and loss-of-function experiments. This evidence concerns the gene LINC00330 and esophageal squamous cell carcinoma.