GFAP and Alzheimer disease: Here we demonstrate the effectiveness of the expanded QUINT workflow to quantify diverse cellular and pathological features in AD-BXD mice (Fig. 1b) by quantifying all nuclei (thionine), neurons (NeuN), microglia (Iba1), astrocytes (GFAP) and amyloid-beta (AB1-42) in customized regions compiled from CCFv3 regions (Fig. 1c, d).