Finally, we analyzed unlabeled polar and non-polar metabolites and noticed that Gls1 deletion with or without RT caused subtle differences at the level of the metabolites (Supplementary Fig. 5a), but RT in Gls1+/+ sarcomas significantly reduced overall metabolite abundance compared to Gls1+/+ sarcomas (Supplementary Fig. 5d). The gene discussed is GLS; the disease is sarcoma.