The CD19+ B-cell cluster in the CLL patient sample showed a clear increase in AKT (pS473) signal in response to anti-IgM stimulation (5 min) compared to both the corresponding unstimulated (0 min) control sample and the stimulated CD19+ B cell cluster in the sample from the healthy donor (Fig. 4b). Here, AKT1 is linked to B-cell chronic lymphocytic leukemia.