On 9/1/2021, a needle biopsy was performed, and IHC staining showed that the HCC components Glypican-3, Arginase-1, Hepatocyte, and Glutamine synthetase (GS) were positive, while the CCA components CK19 and CK7 were positive, and Ki67 showed approximately 40% cell-positive staining. This evidence concerns the gene GPC3 and cholangiocarcinoma.