To assess the diagnostic value of tRF-17-18VBY9M in GC sera, we performed ROC analysis of tRF-17-18VBY9M, CEA, CA199, and CA724 in the sera of 115 patients with GC and 75 healthy donors, and the results revealed that tRF-17-18VBY9M’s AUC was 0.793 (95%CI 0.727–0.859), which was higher than CEA’s AUC of 0.722 (95%CI 0.647–0.896), CA199’s AUC of 0.678 (0.603–0.754), and CA724’s AUC of 0.694 (95%CI 0.620–0.767), suggesting that tRF-17-18VBY9M has good diagnostic potency in GC serum (Fig. 5A). This evidence concerns the gene CEACAM5 and gastric cancer.