To further investigate the role of TBC1Ds on the malignant feature of HCC, we knockout the expression of TBC1D8 and TBC1D14 in Hep3B cells as these two genes play vital function in other types of cancers (Chen et al., 2019; Lu et al., 2022) and showed relative tighter relationship to the prognosis of patients with HCC. This evidence concerns the gene TBC1D8 and hepatocellular carcinoma.