The most important finding was that the mRNA levels of all six TBC1D members--TBC1D1, TBC1D7, TBC1D8, TBC1D9b, TBC1D14 and TBC1D25—were significantly upregulated in HCC tissues and cholangio carcinoma (CHOL) tissues (Fig. 1A and Fig. S1). Here, TBC1D8 is linked to hepatocellular carcinoma.