To further verify the results, the mRNA levels of TBC1Ds were also explored in the UALCN dataset and the expression levels of TBC1D1, TBC1D7, TBC1D8, TBC1D9b, TBC1D14 and TBC1D25 were significantly higher in HCC than in normal liver tissues (Fig. 1B). This evidence concerns the gene TBC1D8 and hepatocellular carcinoma.