To further verify the results, the mRNA levels of TBC1Ds were also explored in the UALCN dataset and the expression levels of TBC1D1, TBC1D7, TBC1D8, TBC1D9b, TBC1D14 and TBC1D25 were significantly higher in HCC than in normal liver tissues (Fig. 1B). The gene discussed is TBC1D1; the disease is hepatocellular carcinoma.