Another animal model experiment revealed that after sheep with 30 min of cerebral ischemia were treated using hypothermia for 3 h, 48 h, and 72 h, and SMI-312-labeled axons and MBP were quantified in the intragyral white matter, the results showed that ischemia was associated with reduced axonal and myelin area fractions, both ischemia—48 h hypothermia and ischemia—72 h hypothermia may improve axonal area fractions and linearity, suggesting that hypothermia may alleviate post-ischemic axonopathy (54). This evidence concerns the gene MBP and ischemia.