To elucidate how D-TLKIVWX might interact with AD-tau fibrils to induce their disassembly, we cryogenically trapped complexes of D-TLKIVWX (X =I, S and R) with AD-tau fibrils at an intermediate 24-h time point with a lower ratio of D-TLKIVWX to tau (estimated 100:1) in comparison with the time-course experiment in Fig. 2a (500 :1). Here, MAPT is linked to Alzheimer disease.